PRDM9 forms a multiprotein complex tethering recombination hotspots to the chromosomal axis
نویسندگان
چکیده
Emil D. Parvanov, Hui Tian, Timothy Billings, Ruth L. Saxl, Rakesh Aithal, Lumir Krejci, Kenneth Paigen, Petko M. Petkov Center for Genome Dynamics, The Jackson Laboratory, Bar Harbor, ME, USA, 04609 Department of Biology, Masaryk University, Brno, Czech Republic National Centre for Biomolecular Research, Masaryk University, Brno, Czech Republic 625 00 Corresponding authors: [email protected] and [email protected]
منابع مشابه
PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis
In mammals, meiotic recombination occurs at 1- to 2-kb genomic regions termed hotspots, whose positions and activities are determined by PRDM9, a DNA-binding histone methyltransferase. We show that the KRAB domain of PRDM9 forms complexes with additional proteins to allow hotspots to proceed into the next phase of recombination. By a combination of yeast-two hybrid assay, in vitro binding, and ...
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تاریخ انتشار 2016